goat anti human igf 2r antibody  (R&D Systems)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Elevation of IGF-2 receptor and the possible underlying implications in end-stage heart failure patients before and after heart transplantation

doi: 10.1111/j.1582-4934.2011.01414.x

Figure Lengend Snippet: Western blot analysis of cardiac IGF-2R. (A) Representative Western blot analysis of cardiac IGF-2R expression. (B) Densitometric quantification of cardiac IGF-2R expression. The IGF-2R protein levels related to the internal standard protein GAPDH were calculated as the relative abundance. Densitometric analyses of the blots showed an apparent increase in IGF-2R in DCM failing hearts (DCM-HF, n = 5) compared with non-failing control hearts (NF, n = 5; ** P < 0.01). Data are presented as mean ± S.D.

Article Snippet: Sections were then incubated with primary antibodies for 1 hr at room temperature and washed in PBS buffer for 10 min., followed by incubation with IgG-peroxidase conjugated secondary antibody (Sigma-Aldrich, St. Louis, MO, USA) for 1 hr at room temperature, washed in PBS buffer for 10 min., and incubated with 0.5 mg/ml diaminobenzidine tetrahydrochloride 2-hydrate plus 0.05% H 2 O 2 for 5 min. Primary antibodies used included goat anti-human IGF-2R antibody (2 μg/ml, Cat. No. AF2447; R&D System Inc., MN, USA) and rabbit anti-human granzyme B polyclonal antibodies (1:150, Cat. No. Z2145; ZETA Corporation, CA, USA).

Techniques: Western Blot, Expressing, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Elevation of IGF-2 receptor and the possible underlying implications in end-stage heart failure patients before and after heart transplantation

doi: 10.1111/j.1582-4934.2011.01414.x

Figure Lengend Snippet: Histopathological analysis of IGF-2R. (A) Representative light microscopic findings in haematoxylin and eosin staining. The normal appearance of myocardial fibres with central nuclei is seen in non-failing control hearts (NF) and interstitial fibrosis replacement is found in DCM failing hearts (DCM-HF). (B) Representative light microscopic IGF-2R immunoreactivity using a monoclonal antibody, which recognizes IGF-2R. Few and weak immunoreactivity of IGF-2R is observed in non-failing control hearts (NF), but more and strong IGF-2R immunoreactivity can be seen in DCM failing hearts (DCM-HF). Arrows indicated the positive staining.

Article Snippet: Sections were then incubated with primary antibodies for 1 hr at room temperature and washed in PBS buffer for 10 min., followed by incubation with IgG-peroxidase conjugated secondary antibody (Sigma-Aldrich, St. Louis, MO, USA) for 1 hr at room temperature, washed in PBS buffer for 10 min., and incubated with 0.5 mg/ml diaminobenzidine tetrahydrochloride 2-hydrate plus 0.05% H 2 O 2 for 5 min. Primary antibodies used included goat anti-human IGF-2R antibody (2 μg/ml, Cat. No. AF2447; R&D System Inc., MN, USA) and rabbit anti-human granzyme B polyclonal antibodies (1:150, Cat. No. Z2145; ZETA Corporation, CA, USA).

Techniques: Staining, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Elevation of IGF-2 receptor and the possible underlying implications in end-stage heart failure patients before and after heart transplantation

doi: 10.1111/j.1582-4934.2011.01414.x

Figure Lengend Snippet: ELISA of serum IGF-2R and serum CD8. (A) The mean level of serum IGF-2R in DCM heart failure patients before heart transplantation (Bef-Htx, n = 11) was significantly higher than that in non-failing control subjects (NF, n = 11). After heart transplantation, serum IGF-2R levels further increased, peaked at the first month, and gradually reduced close to the pre-operative level at the 6th months, but remained to be higher than that in non-failing controls. ** P < 0.01 versus NF. ## P < 0.01 versus Bef-Htx. (B) The mean level of serum CD8 in DCM heart failure patients before heart transplantation (Bef-Htx, n = 11) had no increase when compared with that in non-failing control subjects (NF, n = 11). Mean levels of serum CD8 also had no change at different time point after heart transplantation compared with that before heart transplantation (Bef-Htx). Data are presented as mean ± S.D.

Article Snippet: Sections were then incubated with primary antibodies for 1 hr at room temperature and washed in PBS buffer for 10 min., followed by incubation with IgG-peroxidase conjugated secondary antibody (Sigma-Aldrich, St. Louis, MO, USA) for 1 hr at room temperature, washed in PBS buffer for 10 min., and incubated with 0.5 mg/ml diaminobenzidine tetrahydrochloride 2-hydrate plus 0.05% H 2 O 2 for 5 min. Primary antibodies used included goat anti-human IGF-2R antibody (2 μg/ml, Cat. No. AF2447; R&D System Inc., MN, USA) and rabbit anti-human granzyme B polyclonal antibodies (1:150, Cat. No. Z2145; ZETA Corporation, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Transplantation Assay, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Elevation of IGF-2 receptor and the possible underlying implications in end-stage heart failure patients before and after heart transplantation

doi: 10.1111/j.1582-4934.2011.01414.x

Figure Lengend Snippet: Immunohistochemical detection of IGF-2R and granzyme B-positive T lymphocytes. Representative immunohistochemical staining on the serial sections from biopsies with rejection grade 3 from patients #6 (A) and from biopsies with rejection grade 2 from patient #4 (B) at the 3 rd month after heart transplantation showed coexistence of IGF-2R and granzyme B-positive T lymphocytes, accompanied by mononuclear cell infiltration with myocyte damage in the same sites in haematoxylin and eosin (HE) staining.

Article Snippet: Sections were then incubated with primary antibodies for 1 hr at room temperature and washed in PBS buffer for 10 min., followed by incubation with IgG-peroxidase conjugated secondary antibody (Sigma-Aldrich, St. Louis, MO, USA) for 1 hr at room temperature, washed in PBS buffer for 10 min., and incubated with 0.5 mg/ml diaminobenzidine tetrahydrochloride 2-hydrate plus 0.05% H 2 O 2 for 5 min. Primary antibodies used included goat anti-human IGF-2R antibody (2 μg/ml, Cat. No. AF2447; R&D System Inc., MN, USA) and rabbit anti-human granzyme B polyclonal antibodies (1:150, Cat. No. Z2145; ZETA Corporation, CA, USA).

Techniques: Immunohistochemical staining, Staining, Transplantation Assay